Journal: eLife
Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons
doi: 10.7554/eLife.101533
Figure Lengend Snippet: ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).
Techniques: Expressing, Staining